Bioreactor Lab

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Temperature

Hardware

Aeration

Sparger

Pluronic F-68 shear protectant, 1 g/L

pH

Bicarbonate-buffered medium. CO₂ in the sparge gas lowers pH; 1 M Na₂CO₃ raises it.

Raman & feed

scan / 15 min

Calibrating PLS models…

Agitation

2 × segment blade

Flow view

Perfusion

ATF hollow-fiber filter, 0.2 µm, 0.13 m² (assumed sizing). Medium in on level control; the bleed holds the VCD target.

Harvest

200 L STR · day 14

Depth pods

Single-use disc-stack centrifuge (50–240 L/h) → Millistak+ X0HC depth pods, 1.1 m² each → Millipore Express SHC 0.5/0.2 µm, 0.69 m². Typical, illustrative sizing; the run follows the time warp.

Time warp

Graphics

O₂, CO₂, pH, jacket heat and cell growth equations are ported from Bioreactor-Sim. The blanket, shear, bubbles, mixing and film estimates are rules of thumb.